<gridview   runat="server" id="GridView1">
<columns>
<asp:TemplateField >
<ItemTemplate>
<asp:linkbutton runat="server" id="Link_Button" autogeneratedcolumns="true" >
 </ItemTemplate>
</TemplateField>
</columns>

AutoGeneratedColumns =true;因为你从数据库绑定网格

AutoGeneratedColumns="true";becouse you Bind Grid From Database