您可以直接在 read_csv

You can use names directly in the read_csv

names : 类似数组，默认 None 要使用的列名列表.如果文件不包含标题行，那么你应该明确地传递 header=None

names : array-like, default None List of column names to use. If file contains no header row, then you should explicitly pass header=None

Cov = pd.read_csv("path/to/file.txt", 
                  sep='	', 
                  names=["Sequence", "Start", "End", "Coverage"])