for (int i = 1; i < dataGridView1.RowCount - 1; i++)
{
   Boolean isEmpty = true;
   for(int j=0;j<dataGridView1.Columns.Count; j++)
   {
     if (dataGridView1.Rows[i].Cells[j].Value.ToString() != "" )
      {
          isEmpty = false;
          break;
      }
    }
    if (isEmpty)
    {
       dataGridView1.Rows.RemoveAt(i);
       i--;
    }
 }

将 AllowUsersToAddRow 设为false。顺便说一下，你可能想要指定 DataGridView 而不是 GridView ，因为后者是web控件。

Set AllowUsersToAddRow as false. BTW, you might want to specify DataGridView instead of GridView since latter is web control.