您可以使用 线程 或 多处理.简单例子:

You can use threading or multiprocessing. Simple example:

import Tkinter
import threading

def create_frame():
    MessFrame = Tkinter.Tk()
    MessFrame.geometry('800x400+200+200')
    MessFrame.title('Main Frame')
    Framelabel = Tkinter.Label(MessFrame, text='Text Here', fg= 'red')
    Framelabel.place(x=10,y=10)
    MessFrame.mainloop()


t1 = threading.Thread(target=create_frame)
t1.start()

#continue
print 1234

但是...通常***将 GUI 保留在主线程中.

But...it's generally best to keep the GUI in the main thread.