我有同样的问题，被我逼疯了最后一个小时。我可以证实，史蒂夫的建议，在命令行输入密码的工作 - 所以在我的情况的htpasswd -b的密码文件的用户的密码的伎俩

这里是Apache相关的bug报告。

I am running Apache 2.4 in Windows Server 2008 R2. I am attempting to password protect a subdirectory and successfully did so in Apache 2.0. After upgrading I took Apache's advice and am attempting to put the authentication config in httpd.config. I am allowing the reading of the password file and everything appears to be in order, but when I test it I get the following error:

[Mon Apr 01 19:58:36.438476 2013] [auth_basic:error] [pid 3984:tid 788] [client xxx.yyy.254.2:49253] AH01617: user master: authentication failure for "/restricted/file.zip": Password Mismatch

However, I know that I am sending the correct password. See below for my config, any comments are helpful.

<Directory "C:/www/mydir/restricted">
    #AllowOverride AuthConfig
    #Order allow,deny
    #Allow from all
    AuthType Basic
    AuthName Restricted
    AuthUserFile "C:/www/mydir/passwords/pass"
    Require valid-user
</Directory>  
<Directory "C:/www/mydir">
    Require all granted
</Directory>  
<VirtualHost *:80>
    ServerAdmin admin@.com
    DocumentRoot "C:/www/mydir"
    ServerName "fakeurl.com"
    ErrorLog "C:/www/mydir/logs/error.log"
    CustomLog "C:/www/mydir/logs/accesslog/access.log" common
</VirtualHost>  
<VirtualHost *:80>
    ServerAdmin admin@.com
    DocumentRoot "C:/www/mydir"
    ServerName "www.fakeurl.com"
    ErrorLog "C:/www/mydir/logs/error.log"
    CustomLog "C:/www/mydir/logs/accesslog/access.log" common
</VirtualHost>

I just had the same issue, was driving me nuts for the last hour. I can confirm that Steve's suggestion to enter the password in the command line works - so in my case "htpasswd -b passwordfile user password" did the trick.

Here is the relevant bug report at Apache.