选项1：

您的关键字是：应用程序池身份

您需要为App Pool用户设置一个帐户有权在SQL上创建数据库

http://www.iis.net/learn/manage/configuring-security/application-pool-identities



选项2：

尝试删除Initial Catalog = project;来自你的连接字符串。

Option 1:
Your keyword are: application pool identities
You need to set App Pool user an account have permission to create DB on SQL
http://www.iis.net/learn/manage/configuring-security/application-pool-identities

Option 2:
Give a try to remove "Initial Catalog=project;" from your connection string.