我认为您错过了 CellContentClick 事件，请尝试以下操作：

I think you missed the CellContentClick event, try this:

private void dataGridView1_CellContentClick(object sender, DataGridViewCellEventArgs e)
{          
     if (e.ColumnIndex == dataGridView1.Columns["Your Column Name"].Index) //To check that we are in the right column
     {
          dataGridView1.EndEdit();  //Stop editing of cell.
          if ((bool)dataGridView1.Rows[e.RowIndex].Cells["Your Column Name"].Value)
          {
             //dataGridView1.Columns[3].ReadOnly = true;// for entire column 
               int colIndex = e.ColumnIndex;
               int rowIndex = e.RowIndex;
               dataGridView1.Rows[colIndex].Cells[rowIndex].ReadOnly = true;
          }
    }
}